Regulatory

Part:BBa_K4343122

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)


TEF

Derived from Y. lipolytica, TEF promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be specifically recognized and bound by RNAP.

Some studies indicate that endoglucanase II was successfully expressed in Yarrowia lipolytica under the control of the TEF promoters.

Reference: Boonvitthya, N., Bozonnet, S., Burapatana, V., O’Donohue, M. J., & Chulalaksananukul, W. (2013). Comparison of the heterologous expression of Trichoderma reesei endoglucanase II and cellobiohydrolase II in the yeasts Pichia pastoris and Yarrowia lipolytica. Molecular biotechnology, 54(2), 158-169.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 423


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